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e coli strain jm109  (Zymo Research)


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    Zymo Research e coli strain jm109
    E Coli Strain Jm109, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e+coli+strain+jm109/Femto+Bacterial+DNA+Quantification+Kit/pm41056240-48-4-8
    Average 95 stars, based on 150 article reviews
    e coli strain jm109 - by Bioz Stars, 2026-10
    95/100 stars

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    Generated:

    Article Title: Dysglycemia and the airway microbiome in cystic fibrosis.
    Article Snippet: Samples, reagent-only negative controls, and mock community-positive controls (Zymo Research D6300) were extracted using a protocol optimized for respiratory samples with a magnetic bead-based protocol using the Maxwell HT 96 gDNA Blood Isolation System (Promega) on a KingFisher Flex instrument as previously described (see S1 File) [11]. qPCR microbial load analysis Quantitative PCR (qPCR) targeting the 16S rRNA gene was performed on sputum samples using a TaqMan probe with FAM dye for detection (Thermo Fisher 16S Pan-bacterial Control (Assay ID Ba04230899_s1). .. Bacterial standards generated from E. coli strain JM109 (Zymo Catalog # E2006) were used to create a standard curve for DNA quantification. .. All qPCR reactions were performed in triplicate using the QuantStudio 7 Pro (Life Technologies).

    Article Title: Dysglycemia and the airway microbiome in cystic fibrosis
    Article Snippet: Quantitative PCR (qPCR) targeting the 16S rRNA gene was performed on sputum samples using a TaqMan probe with FAM dye for detection (Thermo Fisher 16S Pan-bacterial Control (Assay ID Ba04230899_s1). .. Bacterial standards generated from E. coli strain JM109 (Zymo Catalog # E2006) were used to create a standard curve for DNA quantification. .. All qPCR reactions were performed in triplicate using the QuantStudio 7 Pro (Life Technologies).

    other:

    Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
    Article Snippet: PCR products were cloned into EcoRV-digested pACYC184 downstream of the tet promoter using Gibson Assembly Master Mix (NEB).

    Article Title: CRISPR-Cas nickase systems, methods and compositions for sequence manipulation in eukaryotes
    Article Snippet: PCR products were cloned into EcoRV-digested pACYC184 downstream of the tet promoter using Gibson Assembly Master Mix (NEB).

    Cloning:

    Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
    Article Snippet: Challenge plasmids containing spacers with PAM sequences (also referred to herein as “CRISPR motif sequences”) were created by ligating hybridized oligos carrying compatible overhangs (Integrated DNA Technology) into BamHI-digested pUC19. .. Cloning for all constructs was performed in E. coli strain JM109 (Zymo Research). pCRISPR-carrying cells were made competent using the Z-Competent E. coli Transformation Kit and Buffer Set (Zymo Research, T3001) according to manufacturer's instructions. .. PCR products were cloned into EcoRV-digested pACYC184 downstream of the tet promoter using Gibson Assembly Master Mix (NEB).

    Construct:

    Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
    Article Snippet: Challenge plasmids containing spacers with PAM sequences (also referred to herein as “CRISPR motif sequences”) were created by ligating hybridized oligos carrying compatible overhangs (Integrated DNA Technology) into BamHI-digested pUC19. .. Cloning for all constructs was performed in E. coli strain JM109 (Zymo Research). pCRISPR-carrying cells were made competent using the Z-Competent E. coli Transformation Kit and Buffer Set (Zymo Research, T3001) according to manufacturer's instructions. .. PCR products were cloned into EcoRV-digested pACYC184 downstream of the tet promoter using Gibson Assembly Master Mix (NEB).

    Transformation Assay:

    Article Title: CRISPR-Cas component systems, methods and compositions for sequence manipulation
    Article Snippet: Challenge plasmids containing spacers with PAM sequences (also referred to herein as “CRISPR motif sequences”) were created by ligating hybridized oligos carrying compatible overhangs (Integrated DNA Technology) into BamHI-digested pUC19. .. Cloning for all constructs was performed in E. coli strain JM109 (Zymo Research). pCRISPR-carrying cells were made competent using the Z-Competent E. coli Transformation Kit and Buffer Set (Zymo Research, T3001) according to manufacturer's instructions. .. PCR products were cloned into EcoRV-digested pACYC184 downstream of the tet promoter using Gibson Assembly Master Mix (NEB).



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    Large-fragment deletion in E. coli MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.

    Journal: Applied and Environmental Microbiology

    Article Title: A recombineering-based platform for high-throughput genomic editing in Escherichia coli

    doi: 10.1128/aem.00193-25

    Figure Lengend Snippet: Large-fragment deletion in E. coli MG1655 and E. coli Nissle 1917. ( A ) Genomic editing tests of sequence deletion of different lengths in MG1655. ( B ) E. coli MG1655 genomic editing results of sequence deletion of different sizes. ( C ) Deletion of E. coli MG1655 large fragments in different genomic locations. ( D ) Genomic editing tests of sequence deletion of different lengths in EcN. ( E ) EcN genomic editing results of sequence deletion of different sizes. (F through H) The sequencing results of 10 kb, 22 kb, and 58 kb fragment deletions.

    Article Snippet: E. coli strains JM109 (ATCC 53323), MG1655 (ATCC 47076), JW128 , Nissle 1917 , and DH10B served as genetic materials in genomic editing experiments.

    Techniques: Sequencing